Emerin Rekombinanter Antikörper

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• KD/KO Validated

Emerin Rekombinant Antikörper für WB, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra)

Konjugation

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

230089B12

Kat-Nr. : CL750-82888-3

Synonyme

EMD, STA, LEMD5, EDMD, 230089B12

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  WB analysis using CL750-82888-3

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-82888-3 (Emerin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  FC experiment of HEK-293T using CL750-82888-3

  1x10^6 HEK-293T cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated Emerin Recombinant antibody (CL750-82888-3, Clone:230089B12)(red), or 0.4 ug Isotype Control (CL750-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HEK-293T-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL750-82888-3 bindet in WB, FC (Intra) Emerin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Emerin fusion protein Ag0236
• Vollständiger Name: emerin
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 30-35 kDa
• GenBank-Zugangsnummer: BC000738
• Gene symbol: EMD
• Gene ID (NCBI): 2010
• Konjugation: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 755 nm / 780 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Emerin (Emery-Dreifuss muscular dystrophy) (EMD or EDMD), a serine-rich nuclear membrane protein, is a member of the nuclear lamina-associated protein family. EMD may mediate membrane anchorage to the cytoskeleton by stabilizing and promoting the formation of a nuclear actin cortical network. Defects in EMD gene are the cause of Emery-Dreifuss muscular dystrophy type 1 (EDMD1), a degenerative myopathy characterized by weakness and atrophy of muscle without involvement of the nervous system, early contractures of the elbows Achilles tendons and spine, and cardiomyopathy associated with cardiac conduction defects. EMD inhibits beta-catenin activity by preventing its accumulation in the nucleus and is involved in HIV-1 infection.