EMILIN1 Polyklonaler Antikörper

EMILIN1 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF,ELISA

Konjugation

Unkonjugiert

Publikationen

1

Rezensionen (1)

Kat-Nr. : 10643-1-AP

Synonyme

EMI, EMILIN, EMILIN 1, EMILIN1, gp115

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Galerie der Validierungsdaten

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  WB analysis of human kidney using 10643-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 10643-1-AP (EMILIN1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 10643-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10643-1-AP (EMILIN1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10643-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10643-1-AP (EMILIN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 10643-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 10643-1-AP (EMILIN1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 10643-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 10643-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 10643-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 10643-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 10643-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 10643-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 10643-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 10643-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 10643-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 10643-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 10643-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 10643-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 10643-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 10643-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 10643-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 10643-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 10643-1-AP (EMILIN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Nierengewebe, humanes Hirngewebe, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Eierstockgewebe, humanes Hautgewebe, humanes Herzgewebe, humanes Hodengewebe, humanes Milzgewebe, humanes Nierengewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

10643-1-AP bindet in WB, IHC, IF,ELISA EMILIN1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EMILIN1 fusion protein Ag1016
• Vollständiger Name: elastin microfibril interfacer 1
• Berechnetes Molekulargewicht: 107 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC007530
• Gene symbol: EMILIN1
• Gene ID (NCBI): 11117
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EMILIN1, also named as EMILIN, EMILIN-1, gp115 and EMI, may be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved not only in the formation of the elastic fiber, but also in the processes that regulate vessel assembly. It has cell adhesive capacity. EMILIN1 is a negative regulator of the transforming growth factor-beta (TGF-beta) signaling, which is involved in blood pressure (BP) homeostasis (PMID:20186130). EMILIN1 may play a key role in hypertensive vascular remodeling. TGFβ proteins are the main regulators of blood vessel development and maintenance, and EMILIN1 inhibits TGFβ signaling by binding specifically to the proTGFβ precursor and preventing its maturation by furin convertases in the extracellular space (PMID:19922630).

Publikationen

Species	Application	Title
human	WB	Cancer Res; Comparative oncogenomics identifies PSMB4 and SHMT2 as potential cancer driver genes.; Authors - Genee Y Lee

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ryan (Verified Customer) (06-15-2018)

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: zebrafish retina

EMILIN1 Antibody Immunohistochemistry validation (1:100 dilution) in zebrafish retina (Cat no:10643-1-AP)

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