EMILIN1 Monoklonaler Antikörper

EMILIN1 Monoklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2A5C8

Kat-Nr. : 60047-1-PBS

Synonyme

Elastin microfibril interface-located protein 1, EMI, EMILIN, EMILIN 1, EMILIN-1

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  WB analysis of human colon using 60047-1-Ig (same clone as 60047-1-PBS)

  Untreated and PNGase F-treated lysates of human colon tissue were subjected to SDS PAGE followed by western blot with 60047-1-Ig (EMILIN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 60047-1-PBS in a different storage buffer formulation.

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  WB analysis of human colon using 60047-1-Ig (same clone as 60047-1-PBS)

  human colon tissue were subjected to SDS PAGE followed by western blot with 60047-1-Ig (EMILIN1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60047-1-PBS in a different storage buffer formulation.

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  WB analysis of human rectum using 60047-1-Ig (same clone as 60047-1-PBS)

  human rectum tissue were subjected to SDS PAGE followed by western blot with 60047-1-Ig (EMILIN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60047-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 60047-1-Ig (same clone as 60047-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60047-1-Ig (EMILIN1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60047-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 60047-1-Ig (same clone as 60047-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60047-1-Ig (EMILIN1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60047-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 60047-1-Ig (same clone as 60047-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60047-1-Ig (EMILIN1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60047-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 60047-1-Ig (same clone as 60047-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60047-1-Ig (EMILIN1 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60047-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 60047-1-Ig (same clone as 60047-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60047-1-Ig (EMILIN1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60047-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 60047-1-Ig (same clone as 60047-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60047-1-Ig (EMILIN1 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60047-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 60047-1-Ig (same clone as 60047-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60047-1-Ig (EMILIN1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60047-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 60047-1-Ig (same clone as 60047-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60047-1-Ig (EMILIN1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60047-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 60047-1-Ig (same clone as 60047-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60047-1-Ig (EMILIN1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60047-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 60047-1-Ig (same clone as 60047-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60047-1-Ig (EMILIN1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60047-1-PBS in a different storage buffer formulation.

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  IF Staining of human ovary tumor using 60047-1-Ig (same clone as 60047-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using EMILIN1 antibody (60047-1-Ig, Clone: 2A5C8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60047-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human ovary tumor using 60047-1-Ig (same clone as 60047-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using EMILIN1 antibody (60047-1-Ig, Clone: 2A5C8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60047-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60047-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA EMILIN1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: EMILIN1 fusion protein Ag1016
• Vollständiger Name: elastin microfibril interfacer 1
• Berechnetes Molekulargewicht: 107 kDa
• Beobachtetes Molekulargewicht: 120-130 kDa
• GenBank-Zugangsnummer: BC007530
• Gene symbol: EMILIN1
• Gene ID (NCBI): 11117
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EMILIN1, also named as EMILIN, EMILIN-1, gp115 and EMI, may be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved not only in the formation of the elastic fiber, but also in the processes that regulate vessel assembly. It has cell adhesive capacity. EMILIN1 is a negative regulator of the transforming growth factor-beta (TGF-beta) signaling, which is involved in blood pressure (BP) homeostasis (PMID:20186130). EMILIN1 may play a key role in hypertensive vascular remodeling. TGFβ proteins are the main regulators of blood vessel development and maintenance, and EMILIN1 inhibits TGFβ signaling by binding specifically to the proTGFβ precursor and preventing its maturation by furin convertases in the extracellular space (PMID:19922630).