EMR2 Rekombinanter Antikörper

EMR2 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

242042E3

Kat-Nr. : 84608-5-RR

Synonyme

EGF-like module receptor 2, EGF like module receptor 2, CD312, Adhesion G protein-coupled receptor E2, ADGRE2

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  WB analysis using 84608-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84608-5-RR (EMR2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84608-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84608-5-RR against Human EMR2 were performed. The affinity constant is 1.45 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, L02-Zellen, THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84608-5-RR bindet in WB, ELISA EMR2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: egf-like module containing, mucin-like, hormone receptor-like 2
• Berechnetes Molekulargewicht: 90 kDa
• Beobachtetes Molekulargewicht: 75~90 kDa
• GenBank-Zugangsnummer: NM_013447.3
• Gene symbol: EMR2
• Gene ID (NCBI): 30817
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EMR2 (Epidermal Growth Factor-like module-containing Mucin-like Hormone Receptor 2), also known as ADGRE2, is a member of the adhesion-GPCR family that contains extracellular EGF-like domains. EMR2 contains a total of five tandem EGF-like domains and expresses similar protein isoforms consisting of various numbers of EGF-like domains as a result of alternative RNA splicing (PMID: 10903844). EMR2 is restricted to myeloid cells including monocytes, macrophages, dendritic cells and granulocytes28 and its expression is highly regulated during monocyte/macrophage differentiation (PMID: 31969668).The ligation of EMR2 could increase neutrophil adhesion, migration and anti-microbial mediator production and could enhance the systemic inflammation (PMID: 27905560).