ENC1 Polyklonaler Antikörper

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• KD/KO Validated

ENC1 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Rezensionen (1)

Kat-Nr. : 15007-1-AP

Synonyme

CCL28, ENC 1, ENC1, Kelch like protein 37, KLHL35, KLHL37, NRPB, Nuclear matrix protein NRP/B, p53 induced gene 10 protein, PIG10, TP53I10

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• 

  WB analysis of SH-SY5Y using 15007-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15007-1-AP (ENC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 15007-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 15007-1-AP (ENC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 15007-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 15007-1-AP (ENC1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 15007-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15007-1-AP (ENC1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15007-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15007-1-AP (ENC1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 15007-1-AP

  IP result of anti-ENC1 (IP:15007-1-AP, 4ug; Detection:15007-1-AP 1:1000) with mouse brain tissue lysate 2640ug.

• 

  IHC staining of human pancreas using 15007-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 15007-1-AP (ENC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 15007-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 15007-1-AP (ENC1 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, humanes Lungengewebe, MCF-7-Zellen, Maushirngewebe, Neuro-2a-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

15007-1-AP bindet in WB, IHC, IP, CoIP, ELISA ENC1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ENC1 fusion protein Ag6607
• Vollständiger Name: ectodermal-neural cortex (with BTB-like domain)
• Berechnetes Molekulargewicht: 66 kDa
• Beobachtetes Molekulargewicht: 67 kDa, 57 kDa
• GenBank-Zugangsnummer: BC000418
• Gene symbol: ENC1
• Gene ID (NCBI): 8507
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	CoIP	Int J Mol Sci; APEX2-Mediated Proximity Labeling Resolves the DDIT4-Interacting Proteome.; Authors - Marianna Naki
human	WB, IHC	Front Cell Dev Biol; ENC1 Facilitates Colorectal Carcinoma Tumorigenesis and Metastasis via JAK2/STAT5/AKT Axis-Mediated Epithelial Mesenchymal Transition and Stemness.; Authors - Ying Cui ; KD Validated
human	WB,IP,IHC,CoIP	Mol Carcinog; ENC1 Promotes the Malignant Progression and Metastasis by Suppressing TRIM21 Mediated Vimentin Degradation in Wilms Tumor; Authors - Zhiyi Lu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hee (Verified Customer) (08-16-2019)

Very good

• Applications: Western Blot,