Endoglin/CD105 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Endoglin/CD105 Polyklonal Antikörper für FC, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

25

Kat-Nr. : 10862-1-AP

Synonyme

CD105, END, Endoglin, ENG, HHT1, ORW, ORW1

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Galerie der Validierungsdaten

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  WB analysis using 10862-1-AP

  human placenta tissue was subjected to SDS PAGE followed by western blot with 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 10862-1-AP

  IP Result of anti-Endoglin/CD105 (IP:10862-1-AP, 3ug; Detection:10862-1-AP 1:300) with mouse lung tissue lysate 4000ug.

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  IHC staining of human breast cancer using 10862-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10862-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 10862-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 10862-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 10862-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 10862-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10862-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10862-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of THP-1 using 10862-1-AP

  1X10^6 THP-1 cells were intracellularly stained with 0.4 ug Anti-Human Endoglin/CD105 (10862-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe
• Erfolgreiche IP: Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Leberkarzinomgewebe, humanes Plazenta-Gewebe, Rattennierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 8 publications below
• IF: See 9 publications below
• FC: See 1 publications below

Produktinformation

10862-1-AP bindet in WB, IP, IHC, IF, FC, ELISA Endoglin/CD105 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Endoglin/CD105 fusion protein Ag1287
• Vollständiger Name: endoglin
• Berechnetes Molekulargewicht: 71 kDa
• Beobachtetes Molekulargewicht: 75-90 kDa, 170-180 kDa
• GenBank-Zugangsnummer: BC014271
• Gene symbol: ENG
• Gene ID (NCBI): 2022
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 90-95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)

Publikationen

Species	Application	Title
human	WB	Cancers (Basel); WISP2/CCN5 Suppresses Vasculogenic Mimicry through Inhibition of YAP/TAZ Signaling in Breast Cancer Cells.; Authors - Nathalie Ferrand
human	WB	Int J Biol Sci; Roquin2 suppresses breast cancer progression by inhibiting tumor angiogenesis via selectively destabilizing proangiogenic factors mRNA.; Authors - Meicen Zhou
human	WB	Biomedicines; Endoglin Modulates TGFβR2 Induced VEGF and Proinflammatory Cytokine Axis Mediated Angiogenesis in Prolonged DEHP-Exposed Breast Cancer Cells.; Authors - Mahendra Jadhao ; KD Validated
human	IHC	Mol Hum Reprod; Oxidative stress-induced S100B protein from placenta and amnion affects soluble Endoglin release from endothelial cells.; Authors - Tskitishvili E E
rat	IF	World J Stem Cells; Quercetin ameliorates oxidative stress-induced senescence in rat nucleus pulposus-derived mesenchymal stem cells via the miR-34a-5p/SIRT1 axis; Authors - Wen-Jie Zhao
human,mouse	WB,IHC	Exp Cell Res; Human umbilical cord mesenchymal stem cells regulate CD54 and CD105 in vascular endothelial cells and suppress inflammation in Kawasaki disease.; Authors - Tao Chen

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