Endoglin/CD105 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Endoglin/CD105 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(55)

Kat-Nr. : 10862-1-AP

Synonyme

CD105, END, Endoglin, ENG, HHT1

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  WB analysis of HeLa using 10862-1-AP

  WB result of Endoglin/CD105 antibody (10862-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Endoglin/CD105 transfected HeLa cells.

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  WB analysis using 10862-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10862-1-AP

  human placenta tissue was subjected to SDS PAGE followed by western blot with 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 10862-1-AP

  IP result of anti-Endoglin/CD105 (IP:10862-1-AP, 4ug; Detection:10862-1-AP 1:2000) with mouse lung tissue lysate 1760 ug.

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  IHC staining of human breast cancer using 10862-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10862-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 10862-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 10862-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 10862-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 10862-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10862-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10862-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10862-1-AP (Endoglin/CD105 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HUVEC-Zellen, HeLa-Zellen, humanes Plazenta-Gewebe, Mauslungengewebe, Rattenlungengewebe
• Erfolgreiche IP: Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Leberkarzinomgewebe, humanes Plazenta-Gewebe, Rattennierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 21 publications below
• IHC: See 13 publications below
• IF: See 19 publications below
• IP: See 1 publications below

Produktinformation

10862-1-AP bindet in WB, IHC, IF, IP, ELISA Endoglin/CD105 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Endoglin/CD105 fusion protein Ag1287
• Vollständiger Name: endoglin
• Berechnetes Molekulargewicht: 71 kDa
• Beobachtetes Molekulargewicht: 75-90 kDa, 170-180 kDa
• GenBank-Zugangsnummer: BC014271
• Gene symbol: CD105
• Gene ID (NCBI): 2022
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 90-95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)

Publikationen

Species	Application	Title
rat	IF	Nat Commun; Injectable ECM-mimetic dynamic hydrogels abolish ferroptosis-induced post-discectomy herniation through delivering nucleus pulposus progenitor cell-derived exosomes; Authors - Wenkai Wang
human	WB	Dev Cell; Endothelial progenitor cells control remodeling of uterine spiral arteries for the establishment of utero-placental circulation; Authors - Bin Tan
rat		Stem Cell Res Ther; Controlled release of hydrogel-encapsulated mesenchymal stem cells-conditioned medium promotes functional liver regeneration after hepatectomy in metabolic dysfunction-associated steatotic liver disease; Authors - Naoya Kasahara
human	WB	Cancers (Basel); WISP2/CCN5 Suppresses Vasculogenic Mimicry through Inhibition of YAP/TAZ Signaling in Breast Cancer Cells.; Authors - Nathalie Ferrand
human	WB	Int J Biol Sci; Roquin2 suppresses breast cancer progression by inhibiting tumor angiogenesis via selectively destabilizing proangiogenic factors mRNA.; Authors - Meicen Zhou
human	WB	Biomedicines; Endoglin Modulates TGFβR2 Induced VEGF and Proinflammatory Cytokine Axis Mediated Angiogenesis in Prolonged DEHP-Exposed Breast Cancer Cells.; Authors - Mahendra Jadhao ; KD Validated