ENGASE Polyklonaler Antikörper

ENGASE Polyklonal Antikörper für IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31784-1-PBS

Synonyme

EC:3.2.1.96, Cytosolic endo-beta-N-acetylglucosaminidase

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  IHC staining of mouse spleen using 31784-1-AP (same clone as 31784-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31784-1-AP (ENGASE antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31784-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 31784-1-AP (same clone as 31784-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31784-1-AP (ENGASE antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31784-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse thymus using 31784-1-AP (same clone as 31784-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 31784-1-AP (ENGASE antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31784-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse thymus using 31784-1-AP (same clone as 31784-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 31784-1-AP (ENGASE antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31784-1-PBS in a different storage buffer formulation.

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  IF Staining of U-251 using 31784-1-AP (same clone as 31784-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using ENGASE antibody (31784-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 31784-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

31784-1-PBS bindet in IHC, IF/ICC, Indirect ELISA ENGASE und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ENGASE fusion protein Ag34633
• Vollständiger Name: endo-beta-N-acetylglucosaminidase
• Berechnetes Molekulargewicht: 84 kDa
• GenBank-Zugangsnummer: NM_001042573
• Gene symbol: ENGASE
• Gene ID (NCBI): 64772
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Endo-β-N-acetylglucosaminidase (ENGASE) is an enzyme that belongs to glycoside hydrolase (GH) families 18 and 85, and is widely distributed in bacteria, fungi, and higher-order species. ENGASE hydrolyzes the β1-4 linkage of the N, N-diacetylchitobiose (GlcNAcβ1-4GlcNAc) region of the N-linked glycans present on glycoproteins (PMID: 36435009). ENGASE is widely expressed, with higher expression in thymus and spleen (Refer to Uniprot).