ENO1 Rekombinanter Antikörper

ENO1 Rekombinant Antikörper für IF/ICC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

IF/ICC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2C20

Kat-Nr. : CL488-81478

Synonyme

ENO1L1, EC:4.2.1.11, C-myc promoter-binding protein, Alpha-enolase, 2-phospho-D-glycerate hydro-lyase

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  IF Staining of HepG2 using CL488-81478

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 ENO1 antibody (CL488-81478, Clone: 2C20 ) at dilution of 1:200.

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  IF Staining of HepG2 using CL488-81478

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 ENO1 antibody (CL488-81478, Clone: 2C20 ) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-81478 bindet in IF/ICC ENO1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ENO1 fusion protein Ag1692
• Vollständiger Name: enolase 1, (alpha)
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC015641
• Gene symbol: ENO1
• Gene ID (NCBI): 2023
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ENO1, also named as NNE, ENO1L1, MBPB1, MPB1 and MBP1, belongs to the enolase family. ENO1 is a metabolic enzyme involved in the synthesis of pyruvate. It also acts as a plasminogen receptor and mediates the activation of plasmin and extracellular matrix degradation. In tumor cells, ENO1 is up-regulated and supports the Warburg effect; it is expressed at the cell surface, where it promotes cancer invasion, and is subjected to a specific array of post-translational modifications, namely acetylation, methylation and phosphorylation. ENO1 overexpression and post-translational modifications could be of diagnostic and prognostic value in many cancer types. (PMID: 27814656). This antibody is specific to ENO1 and has no cross-reaction with ENO2 and ENO3.