ENO3-specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ENO3-specific Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 55234-1-AP

Synonyme

Beta enolase, ENO3, ENO3-specific, Enolase 3, enolase 3 (beta, muscle), MSE, Muscle specific enolase, Skeletal muscle enolase

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Galerie der Validierungsdaten

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  WB analysis using 55234-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55234-1-AP (ENO3-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 55234-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55234-1-AP (ENO3-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 55234-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55234-1-AP (ENO3-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 55234-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55234-1-AP (ENO3-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 55234-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55234-1-AP (ENO3-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 55234-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55234-1-AP (ENO3-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 55234-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 55234-1-AP (ENO3-specific antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 55234-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 55234-1-AP (ENO3-specific antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 55234-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55234-1-AP (ENO3-specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 55234-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55234-1-AP (ENO3-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 55234-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55234-1-AP (ENO3-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 55234-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55234-1-AP (ENO3-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 55234-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 55234-1-AP (ENO3-specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, Maushirngewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe, humanes Herzgewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Produktinformation

55234-1-AP bindet in WB, IHC, IF, ELISA ENO3-specific und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: enolase 3 (beta, muscle)
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 42-47 kDa
• GenBank-Zugangsnummer: NM_001976
• Gene symbol: ENO3
• Gene ID (NCBI): 2027
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ENO3, also named as MSE, belongs to the enolase family. It appears to have a function in striated muscle development and regeneration. Defects in ENO3 are the cause of glycogen storage disease type 13 (GSD13). This antibody is specific to ENO3. It has no cross reaction to ENO1 and ENO2.

Publikationen

Species	Application	Title
bovine	WB	J Proteomics; Characterization of the liver proteome in dairy cows experiencing negative energy balance at early lactation.; Authors - Turner H Swartz
human	WB	Ann Transl Med; ENO3 promoted the progression of NASH by negatively regulating ferroptosis via elevation of GPX4 expression and lipid accumulation.; Authors - Di Lu ; KD Validated
human	WB,IHC	Med Oncol; ENO3 promotes colorectal cancer progression by enhancing cell glycolysis.; Authors - Jingyu Chen ; KD Validated
	WB	Reprod Domest Anim; Disaccharide combinations and the expression of enolase3 and plasma membrane Ca2+ ATPase isoform in sturgeon sperm cryopreservation.; Authors - M D Xi