ENOSF1 Rekombinanter Antikörper

ENOSF1 Rekombinant Antikörper für WB, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240796D2

Kat-Nr. : 83769-2-PBS

Synonyme

TYMSAS, rTS, L-fuconate dehydratase, enolase superfamily member 1, EC:4.2.1.68

---

• 

  WB analysis using 83769-2-RR (same clone as 83769-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83769-2-RR (ENOSF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83769-2-PBS in a different storage buffer formulation.

• 

  FC experiment of Hela using 83769-2-RR (same clone as 83769-2-PBS)

  1x10^6 Hela cells were intracellularly stained with 0.25 ug ENOSF1 Recombinant antibody (83769-2-RR, Clone:240796D2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83769-2-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00758-1

  Cytometric bead array standard curve of MP00758-1, ENOSF1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83769-1-PBS. Detection antibody: 83769-2-PBS. Standard: Ag34665. Range: 0.625-80 ng/mL.

• 

  Affinity and Kinetic Characterization of 83769-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83769-2-RR against Human ENOSF1 were performed. The affinity constant is below 1 pM.

---

Geprüfte Anwendungen

Produktinformation

83769-2-PBS bindet in WB, FC (Intra), Cytometric bead array, Indirect ELISA ENOSF1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ENOSF1 fusion protein Ag34665
• Vollständiger Name: enolase superfamily member 1
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 49 kDa
• GenBank-Zugangsnummer: BC001285
• Gene symbol: ENOSF1
• Gene ID (NCBI): 55556
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ENOSF1 (Mitochondrial enolase superfamily member 1) is also named as RTS and TYMSAS. The ENOSF1 gene, responsible for encoding the protein mitochondrial enolase superfamily member 1 (ENOF1), exhibits three distinct isoforms. One of these isoforms has been identified as an l-fuconate dehydratase, actively participating in the catabolism of L-fucose, a sugar integral to the carbohydrate composition of cellular glycoproteins (PMID: 38203276). The ENOSF1 transcript has been shown to act as antisense RNA and inhibit TYMS expression (PMID: 35931051).