ENOX2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ENOX2 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Rezensionen (1)

Kat-Nr. : 10423-1-AP

Synonyme

APK1, APK1 antigen, COVA1, ENOX2, tNOX

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 10423-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10423-1-AP (ENOX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10423-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10423-1-AP (ENOX2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10423-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10423-1-AP (ENOX2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 10423-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 10423-1-AP (ENOX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 10423-1-AP

  IP Result of anti-ENOX2 (IP:10423-1-AP, 3ug; Detection:10423-1-AP 1:700) with MCF-7 cells lysate 4000ug.

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  IHC staining of human ovary tumor using 10423-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10423-1-AP (ENOX2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary tumor using 10423-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10423-1-AP (ENOX2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of MCF-7 using 10423-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 10423-1-AP (ENOX2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HeLa-Zellen, Maus-Eierstockgewebe, PC-3-Zellen
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IP: See 2 publications below

Produktinformation

10423-1-AP bindet in WB, IP, IHC, IF, ELISA ENOX2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ENOX2 fusion protein Ag0674
• Vollständiger Name: ecto-NOX disulfide-thiol exchanger 2
• Berechnetes Molekulargewicht: 70 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC019254
• Gene symbol: ENOX2
• Gene ID (NCBI): 10495
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tumor-associated NADH oxidase (ENOX2) as a member of a family of growth-related NADH (or hydroquinone) oxidases. It functions as a terminal oxidase of plasma electron transport from cytosolic NAD(P)H via hydroquinones to acceptors at the cell surface. Hydroquinone oxidase activity alternates with a protein disulfide-thiol interchange/oxidoreductase activity which may control physical membrane displacements associated with vesicle budding or cell enlargement. The activities oscillate with a period length of 22 minutes and play a role in control of the ultradian cellular biological clock

Publikationen

Species	Application	Title
human	WB	J Med Chem; Synthesis and characterization of 4,11-diaminoanthra[2,3-b]furan-5,10-diones: tumor cell apoptosis through tNOX-modulated NAD+/NADH ratio and SIRT1.; Authors - Alexander S Tikhomirov
human	IP	Am J Cancer Res; Capsaicin acts through tNOX (ENOX2) to induce autophagic apoptosis in p53-mutated HSC-3 cells but autophagy in p53-functional SAS oral cancer cells.; Authors - Chin-Fang Chang
human,mouse	IP	Am J Cancer Res; Capsaicin exerts therapeutic effects by targeting tNOX-SIRT1 axis and augmenting ROS-dependent autophagy in melanoma cancer cells.; Authors - Atikul Islam
human	WB	Exp Cell Res; Phosphorylation of serine-504 of tNOX (ENOX2) modulates cell proliferation and migration in cancer cells.; Authors - Zeng Zih-Ming ZM ; KD Validated
human	WB	J Agric Food Chem; Capsaicin-mediated tNOX (ENOX2) up-regulation enhances cell proliferation and migration in vitro and in vivo.; Authors - Liu Nei-Chi NC
human	WB	Biochem Biophys Res Commun; RNA interference targeting tNOX attenuates cell migration via a mechanism that involves membrane association of Rac.; Authors - Liu Shan-Chi SC ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Wassim (Verified Customer) (04-22-2019)

This is a western blot analysis of Enox2 in membrane fractions as seen in normal epithelial cells versus abnormal cells. The molecular weight of fractions is indicated above each lane.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Epithelial cells

ENOX2 Antibody Western Blot, validation (1:2000 dilution) in Epithelial cells (Cat no:10423-1-AP)