ENPEP Rekombinanter Antikörper

ENPEP Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

250659C1

Kat-Nr. : 98489-3-PBS

Synonyme

Aminopeptidase A, AP-A, BP-1/6C3 antigen, CD249, EAP

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  FC experiment of mouse bone marrow cells using 98489-3-RR (same clone as 98489-3-PBS)

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse ENPEP Rabbit RecAb (98489-3-RR, Clone:250659C1) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with APC Anti-Mouse CD45R. Cells were not fixed. This data was developed using the same antibody clone with 98489-3-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse bone marrow cells using 98489-3-RR (same clone as 98489-3-PBS)

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse ENPEP Rabbit RecAb (98489-3-RR, Clone: 250659C1) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with APC Anti-Mouse CD45R. Cells were not fixed. This data was developed using the same antibody clone with 98489-3-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

98489-3-PBS bindet in FC ENPEP und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ENPEP fusion protein Eg4233
• Vollständiger Name: glutamyl aminopeptidase
• Berechnetes Molekulargewicht: 108 kDa
• GenBank-Zugangsnummer: NM_007934.3
• Gene symbol: Enpep
• Gene ID (NCBI): 13809
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

ENPEP is the gene encoding the protein glutamyl aminopeptidase, also known as aminopeptidase A (APA), human kidney differentiation antigen (gp160). A type 2 membrane zinc metalloenzyme, APA is a general-purpose protease that primarily serves to cleave the acidic N-terminal aspartate and glutamyl residues of a variety of peptides, most significantly angiotensin 2 (ANG2, ang1-8), cholecystokinin-8 (CCK8), and neurokinin A. The presence of Ca2+ augments both the enzymatic efficiency and specificity of APA, increasing its selectivity towards the aforementioned acidic amino acids. Conversely, in the absence of calcium, APA can bind a wider range of N-terminal neutral and basic amino acids, some of which are cleaved, while others bind without proteolysis.