ENPEP Rekombinanter Antikörper

ENPEP Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

250659C1

Kat-Nr. : 98489-3-RR

Synonyme

Aminopeptidase A, AP-A, BP-1/6C3 antigen, CD249, EAP

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  FC experiment of mouse bone marrow cells using 98489-3-RR

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse ENPEP Rabbit RecAb (98489-3-RR, Clone:250659C1) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with APC Anti-Mouse CD45R. Cells were not fixed.

• 

  FC experiment of mouse bone marrow cells using 98489-3-RR

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse ENPEP Rabbit RecAb (98489-3-RR, Clone: 250659C1) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with APC Anti-Mouse CD45R. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Knochenmarkzellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98489-3-RR bindet in FC ENPEP und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ENPEP fusion protein Eg4233
• Vollständiger Name: glutamyl aminopeptidase
• Berechnetes Molekulargewicht: 108 kDa
• GenBank-Zugangsnummer: NM_007934.3
• Gene symbol: Enpep
• Gene ID (NCBI): 13809
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

ENPEP is the gene encoding the protein glutamyl aminopeptidase, also known as aminopeptidase A (APA), human kidney differentiation antigen (gp160). A type 2 membrane zinc metalloenzyme, APA is a general-purpose protease that primarily serves to cleave the acidic N-terminal aspartate and glutamyl residues of a variety of peptides, most significantly angiotensin 2 (ANG2, ang1-8), cholecystokinin-8 (CCK8), and neurokinin A. The presence of Ca2+ augments both the enzymatic efficiency and specificity of APA, increasing its selectivity towards the aforementioned acidic amino acids. Conversely, in the absence of calcium, APA can bind a wider range of N-terminal neutral and basic amino acids, some of which are cleaved, while others bind without proteolysis.