ENPP2 Monoklonaler Antikörper

ENPP2 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

2A10D9

Kat-Nr. : 68724-1-Ig

Synonyme

AUTOTAXIN, E-NPP 2, EC:3.1.4.39, E NPP 2, ATX X

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  WB analysis of HuH-7 using 68724-1-Ig

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 68724-1-Ig (ENPP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-87 MG using 68724-1-Ig

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 68724-1-Ig (ENPP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68724-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68724-1-Ig (ENPP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Hirngewebe, HuH-7-Zellen, Maushirngewebe, Kaninchenhirngewebe, Rattenhirngewebe, U-87 MG-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

68724-1-Ig bindet in WB, ELISA ENPP2 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• In Publikationen genannte Reaktivität: Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ENPP2 fusion protein Ag5499
• Vollständiger Name: ectonucleotide pyrophosphatase/phosphodiesterase 2
• Berechnetes Molekulargewicht: 99 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC034961
• Gene symbol: ENPP2
• Gene ID (NCBI): 5168
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ENPP2, Autotaxin or Extracellular lysophospholipase D, is a 863 amino acid secreted protein, which is detected in blood plasma (at protein level) (PubMed:12176993, PubMed:26371182). ENPP2 is Predominantly expressed in brain, placenta, ovary, and small intestine. ENPP2 is expressed in a number of carcinomas such as hepatocellular and prostate carcinoma, neuroblastoma and non-small-cell lung cancer. ENPP2 is expressed in body fluids such as plasma, cerebral spinal fluid (CSF), saliva, follicular and amniotic fluids. The calculated molecular weight of ENPP2 is about 100 kDa (PMID: 22301782), but modified ENPP2 is 120 kDa (PMID: 19808661). ENPP2 Hydrolyzes lysophospholipids to produce the signaling molecule lysophosphatidic acid (LPA) in extracellular fluids (PubMed:15769751, PubMed:26371182, PubMed:27754931).

Publikationen

Species	Application	Title
rat	WB	J Pharm Pharmacol; Renoprotective effect of liraglutide on diabetic nephropathy by modulation of Krüppel-like transcription factor 5 expression in rats; Authors - Anfal F Bin Dayel