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ENPP2 Monoklonaler Antikörper

ENPP2 Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2A10D9

Kat-Nr. : 68724-1-PBS

Synonyme

AUTOTAXIN, E-NPP 2, EC:3.1.4.39, E NPP 2, ATX X

Filter:
  • Western Blot (WB) analysis of HuH-7 cells using ENPP2 Monoclonal antibody (68724-1-Ig)

    WB analysis of HuH-7 using 68724-1-Ig (same clone as 68724-1-PBS)

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 68724-1-Ig (ENPP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68724-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-87 MG cells using ENPP2 Monoclonal antibody (68724-1-Ig)

    WB analysis of U-87 MG using 68724-1-Ig (same clone as 68724-1-PBS)

    U-87 MG cells were subjected to SDS PAGE followed by western blot with 68724-1-Ig (ENPP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68724-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ENPP2 Monoclonal antibody (68724-1-Ig)

    WB analysis using 68724-1-Ig (same clone as 68724-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68724-1-Ig (ENPP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68724-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

68724-1-PBS bindet in WB, Indirect ELISA ENPP2 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

Getestete Reaktivität Hausschwein, human, Kaninchen, Maus, Ratte
Wirt / Isotyp Maus / IgG2a
Klonalität Monoklonal
Typ Antikörper
Immunogen ENPP2 fusion protein Ag5499
Vollständiger Name ectonucleotide pyrophosphatase/phosphodiesterase 2
Berechnetes Molekulargewicht 99 kDa
Beobachtetes Molekulargewicht 100-110 kDa
GenBank-ZugangsnummerBC034961
Gene symbol ENPP2
Gene ID (NCBI) 5168
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ENPP2, Autotaxin or Extracellular lysophospholipase D, is a 863 amino acid secreted protein, which is detected in blood plasma (at protein level) (PubMed:12176993, PubMed:26371182). ENPP2 is Predominantly expressed in brain, placenta, ovary, and small intestine. ENPP2 is expressed in a number of carcinomas such as hepatocellular and prostate carcinoma, neuroblastoma and non-small-cell lung cancer. ENPP2 is expressed in body fluids such as plasma, cerebral spinal fluid (CSF), saliva, follicular and amniotic fluids. The calculated molecular weight of ENPP2 is about 100 kDa (PMID: 22301782), but modified ENPP2 is 120 kDa (PMID: 19808661). ENPP2 Hydrolyzes lysophospholipids to produce the signaling molecule lysophosphatidic acid (LPA) in extracellular fluids (PubMed:15769751, PubMed:26371182, PubMed:27754931).