CD39/ENTPD1 Rekombinanter Antikörper

CD39/ENTPD1 Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241292A1

Kat-Nr. : 84235-4-PBS

Synonyme

ENTPD1, 241292A1, ATP diphosphohydrolase, ATPDase, ATP-DPH

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  WB analysis of human placenta using 84235-4-RR (same clone as 84235-4-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 84235-4-RR (ENTPD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84235-4-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 84235-4-RR (same clone as 84235-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84235-4-RR (ENTPD1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84235-4-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 84235-4-RR (same clone as 84235-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84235-4-RR (ENTPD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84235-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 84235-4-RR (same clone as 84235-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84235-4-RR (ENTPD1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84235-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 84235-4-RR (same clone as 84235-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84235-4-RR (ENTPD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84235-4-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

84235-4-PBS bindet in WB, IHC, Indirect ELISA CD39/ENTPD1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: ectonucleoside triphosphate diphosphohydrolase 1
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC047664
• Gene symbol: CD39
• Gene ID (NCBI): 953
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ENTPD1(Ectonucleoside triphosphate diphosphohydrolase 1) is also named CD39, NTPDase 1, and Ecto-ATPDase 1 and belongs to the GDA1/CD39 NTPase family. In the nervous system, it can hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. It is a noncovalent tetrameric protein and detergent inhibition of enzymatic activity is caused by dissociation of ectoapyrase tetramers into monomers(PMID: 9733785 ). And it is a 70- to 100-kDa protein that is heavily N-glycosylated(PMID:7930580). This protein has 6 glycosylation sites and 3 isoforms(58 kDa, 59 kDa, and 34 kDa) produced by alternative splicing.