ENTPD5 Polyklonaler Antikörper

ENTPD5 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26746-1-AP

Synonyme

CD39 antigen-like 4, CD39L4, EC:3.6.1.6, ER UDPase, ER-UDPase

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  WB analysis of mouse liver using 26746-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 26746-1-AP (ENTPD5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 26746-1-AP

  IP result of anti-ENTPD5 (IP:26746-1-AP, 4ug; Detection:26746-1-AP 1:300) with mouse liver tissue lysate 6000ug.

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  IHC staining of human liver using 26746-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26746-1-AP (ENTPD5 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 26746-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26746-1-AP (ENTPD5 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

26746-1-AP bindet in WB, IHC, IP, ELISA ENTPD5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ENTPD5 fusion protein Ag25022
• Vollständiger Name: ectonucleoside triphosphate diphosphohydrolase 5
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC130485
• Gene symbol: ENTPD5
• Gene ID (NCBI): 957
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ectonucleoside triphosphate diphosphohydrolases (NTPDases) are enzymes that are involved in the degradation of di- and triphosphate nucleotides, with important implications in several biological processes that underlie a number of physiological and pathological conditions, including cancer [PMID: 34272651]. ENTPD5 gene, hydrolyzes UDP to UMP and is mainly localized inside the cell, where it plays a critical role in the N-glycosylation and proper folding of proteins in the endoplasmic reticulum [PMID: 21074248]. It can also be secreted into the extracellular space and can work as a soluble enzyme upon cleavage of a signal peptide [PMID: 15698960]. NTPDase5 is often deregulated in cancer, likely as a consequence of TP53 mutations, where it promotes and favours tumour progression and metastasis [PMID: 27956623]. Furthermore, short NPTDase5 peptides were detected in normal and tumour cell lines, recalling the mt-PCPH oncoprotein, a truncated form of the enzyme originating from a single-nucleotide deletion described in Syrian hamster and lacking enzymatic activity [PMID: 27956623].