p300 Polyklonaler Antikörper

p300 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 20695-1-AP

Synonyme

EP300, E1A-associated protein p300, EC:2.3.1.-, EC:2.3.1.48, Histone butyryltransferase p300

---

• 

  WB analysis using 20695-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20695-1-AP (p300 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20695-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20695-1-AP (p300 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, Jurkat-Zellen, MCF-7-Zellen, PC-3-Zellen, RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

20695-1-AP bindet in WB, IHC, IF, ELISA p300 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: E1A binding protein p300
• Berechnetes Molekulargewicht: 264 kDa
• Beobachtetes Molekulargewicht: 300 kDa
• GenBank-Zugangsnummer: NM_001429
• Gene symbol: EP300
• Gene ID (NCBI): 2033
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

p300 is a 300 kDa global transcriptional coactivator with histone acetyltransferase (HAT) activity. p300 binds to chromosomal histones and modulates the chromatin conformation by acetylation of its histones, leading to activation of various gene transcriptions. p300 plays a critical role in DNA repair synthesis after DNA damage induced by ultraviolet or radiation. p300 is overexpressed in various types of cancer, which is associated with worse patient outcomes (PMID: 27322077).

Publikationen

Species	Application	Title
human	WB	Cell Metab; Targeting gut microbial nitrogen recycling and cellular uptake of ammonium to improve bortezomib resistance in multiple myeloma; Authors - Yinghong Zhu
mouse,human	WB,IHC	Exp Cell Res; The Ku protein family regulates hyperglycemia-induced vascular endothelial cell inflammation by modulating P300 levels; Authors - Qinqin Cai
human	WB	Cancer Lett; NEAT1 Promotes the Perineural Invasion of Pancreatic Cancer via the E2F1/GDNF Axis; Authors - Jingtao Gu
human	WB	Commun Biol; Exosome-derived circ-001422 promotes tumor-associated macrophage M2 polarization to accelerate the progression of glioma; Authors - Wenpeng Cao
human	WB,IHC	Adv Sci (Weinh); UGDH Lactylation Aggravates Osteoarthritis by Suppressing Glycosaminoglycan Synthesis and Orchestrating Nucleocytoplasmic Transport to Activate MAPK Signaling; Authors - Weiren Lan
mouse	WB,IF	Cell Biochem Biophys; The Paeonol of Total Glucosides of White Peony Regulates the Differentiation of CD4+Treg Cells through the EP300/Foxp3 Axis to Relieve Pulmonary Fibrosis in Mice; Authors - Muyun Yan