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EP400 Polyklonaler Antikörper

EP400 Polyklonal Antikörper für IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13858-1-PBS

Synonyme

CAGH32, E1A-binding protein p400, EC:3.6.4.-, EP 400, hDomino

Filter:
  • Immunoprecipitation (IP) experiment of HEK-293 cells using EP400 Polyclonal antibody (13858-1-AP)

    IP experiment of HEK-293 using 13858-1-AP (same clone as 13858-1-PBS)

    IP result of anti-EP400 (IP:13858-1-AP, 4ug; Detection:13858-1-AP 1:500) with HEK-293 cells lysate 1440 ug. This data was developed using the same antibody clone with 13858-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using EP400 Polyclonal antibody (13858-1-AP)

    IHC staining of human colon using 13858-1-AP (same clone as 13858-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13858-1-AP (EP400 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13858-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EP400 Polyclonal antibody (13858-1-AP)

    IF Staining of HeLa using 13858-1-AP (same clone as 13858-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EP400 antibody (13858-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 13858-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using EP400 Polyclonal antibody (13858-1-AP)

    IF Staining of U2OS using 13858-1-AP (same clone as 13858-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using EP400 antibody (13858-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 13858-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

13858-1-PBS bindet in IHC, IF/ICC, IP, Indirect ELISA EP400 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen EP400 fusion protein Ag4832
Vollständiger Name E1A binding protein p400
Berechnetes Molekulargewicht 3160 aa, 344 kDa
Beobachtetes Molekulargewicht344 kDa
GenBank-ZugangsnummerBC037208
Gene symbol EP400
Gene ID (NCBI) 57634
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

E1A binding protein p400 (EP400) is a SWR1-class ATP-dependent chromatin remodeling protein originally identified as an interacting partner for the adenovirus E1A protein required for viral replication and cellular transformation. EP400 plays a crucial role in promoting double-strand break (DSB) repair through homologous recombination (PMID: 26669263, 37922899). Depletion of Ep400 in oocytes led to accumulated DSBs, causing abnormal aggregation and dysfunction of cytoplasmic organelles, resulting in impaired oocyte development and declined oocyte quality (PMID: 38493496).