EPAC1 Polyklonaler Antikörper

EPAC1 Polyklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

hamster, human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12572-1-PBS

Synonyme

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  WB analysis of CHO using 12572-1-AP (same clone as 12572-1-PBS)

  CHO cells were subjected to SDS PAGE followed by western blot with 12572-1-AP (EPAC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12572-1-PBS in a different storage buffer formulation.

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  WB analysis using 12572-1-AP (same clone as 12572-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12572-1-AP (EPAC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12572-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 12572-1-AP (same clone as 12572-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12572-1-AP (EPAC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12572-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 12572-1-AP (same clone as 12572-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12572-1-AP (EPAC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12572-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 12572-1-AP (same clone as 12572-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 12572-1-AP (EPAC1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12572-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse testis using 12572-1-AP (same clone as 12572-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 12572-1-AP (EPAC1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12572-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12572-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA EPAC1 und zeigt Reaktivität mit hamster, human, Maus, Ratten

• Getestete Reaktivität: hamster, human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EPAC1 fusion protein Ag3240
• Vollständiger Name: Rap guanine nucleotide exchange factor (GEF) 3
• Berechnetes Molekulargewicht: 881 aa, 99 kDa
• Beobachtetes Molekulargewicht: 99-104 kDa
• GenBank-Zugangsnummer: BC017728
• Gene symbol: EPAC1/RAPGEF3
• Gene ID (NCBI): 10411
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EPAC1 functions as a guanine nucleotide exchange factor (GEF), activating small GTPases like Rap1 and Rap2, leading to diverse cellular response. Epac proteins are cAMP sensors that regulate several pivotal cellular processes, including calcium handling, cell proliferation, cell survival, cell differentiation, cell polarization, cell adhesion events, gene transcription, secretion, ion transport, and neuronal signaling. It is a potential target for therapeutic intervention, such as metabolic disorders, cardiovascular diseases (PMID: 20007405, PMID: 20421979). EPAC1 also enhances brown fat growth and beige adipogenesis (PMID: 38195707).