HIF2α/EPAS1 Rekombinanter Antikörper

HIF2α/EPAS1 Rekombinant Antikörper für WB, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240736F2

Kat-Nr. : 83790-1-PBS

Synonyme

EPAS1, 240736F2, Basic-helix-loop-helix-PAS protein MOP2, bHLHe73, Class E basic helix-loop-helix protein 73

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  WB analysis of A549 using 83790-1-RR (same clone as 83790-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 83790-1-RR (EPAS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83790-1-PBS in a different storage buffer formulation.

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  WB analysis of rat kidney using 83790-1-RR (same clone as 83790-1-PBS)

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 83790-1-RR (EPAS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83790-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83790-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83790-1-RR against Human HIF2α/EPAS1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83790-1-PBS bindet in WB, Cytometric bead array, Indirect ELISA HIF2α/EPAS1 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: HIF2α/EPAS1 fusion protein Ag15199
• Vollständiger Name: endothelial PAS domain protein 1
• Berechnetes Molekulargewicht: 96 kDa
• Beobachtetes Molekulargewicht: 100-120 kDa
• GenBank-Zugangsnummer: BC051338
• Gene symbol: HIF2α
• Gene ID (NCBI): 2034
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HIF2A, also named as EPAS1, is a 870 amino acid protein, which is expressed in most tissues, with highest levels in placenta, lung and heart. HIF2A colocalizes with HIF3A in the nucleus and speckles. HIF2A as a transcription factor involves in the induction of oxygen regulated genes. HIF2A binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. HIF2A regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. HIF2A may also play a role in the formation of the endothelium that gives rise to the blood brain barrier. The calculated molecular weight of HIF2A is 96 kDa, but In normoxia, HIF2A is probably hydroxylated on Pro-405 and Pro-531 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization. The modified HIf2A is about 100-120 kDa.