EPB41L2 Polyklonaler Antikörper

EPB41L2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15437-1-AP

Synonyme

Erythrocyte membrane protein band 4.1-like 2, EPB41 L2, Band 4.1-like protein 2, Band 4.1 like protein 2, 4.1G

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  WB analysis of HeLa using 15437-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15437-1-AP (EPB41L2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 15437-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15437-1-AP (EPB41L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 15437-1-AP

  IP result of anti-EPB41L2 (IP:15437-1-AP, 4ug; Detection:15437-1-AP 1:4000) with HEK-293 cells lysate 1610 ug.

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  IHC staining of human ovary tumor using 15437-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15437-1-AP (EPB41L2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 15437-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15437-1-AP (EPB41L2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 15437-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using EPB41L2 antibody (15437-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  IF Staining of HeLa using 15437-1-AP

  Immunofluorescent analysis of HeLa cells using 15437-1-AP (EPB41L2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

15437-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA EPB41L2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EPB41L2 fusion protein Ag3647
• Vollständiger Name: erythrocyte membrane protein band 4.1-like 2
• Berechnetes Molekulargewicht: 112 kDa
• Beobachtetes Molekulargewicht: 130-150 kDa
• GenBank-Zugangsnummer: BC034718
• Gene symbol: EPB41L2
• Gene ID (NCBI): 2037
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EPB41L2, also known as 4.1G, is a member of the protein 4.1 family, which generally serves as adaptors linking transmembrane proteins to the cytoskeleton(PMID: 21482674).