EPB41L4B Rekombinanter Antikörper

EPB41L4B Rekombinant Antikörper für WB, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242186G4

Kat-Nr. : 84732-2-PBS

Synonyme

242186G4, Band 4.1 like protein 4B, CG1, EHM2, FERM containing protein CG1

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  WB analysis using 84732-2-RR (same clone as 84732-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84732-2-RR (EPB41L4B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84732-2-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01525-2

  Cytometric bead array standard curve of MP01525-2, EPB41L4B Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84732-2-PBS. Detection antibody: 84732-3-PBS. Standard: Ag36353. Range: 0.781-100 ng/mL.

• 

  Cytometric bead array standard curve of MP01525-1

  Cytometric bead array standard curve of MP01525-1, EPB41L4B Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84732-2-PBS. Detection antibody: 84732-1-PBS. Standard: Ag36353. Range: 0.781-100 ng/mL.

• 

  Affinity and Kinetic Characterization of 84732-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84732-2-RR against Human EPB41L4B were performed. The affinity constant is 6.86 nM.

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Geprüfte Anwendungen

Produktinformation

84732-2-PBS bindet in WB, Cytometric bead array, Indirect ELISA EPB41L4B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: EPB41L4B fusion protein Ag36353
• Vollständiger Name: erythrocyte membrane protein band 4.1 like 4B
• Berechnetes Molekulargewicht: 99kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: NM_00138562
• Gene symbol: EPB41L4B
• Gene ID (NCBI): 54566
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The 4.1 proteins, encoded by the EPB41 (erythrocyte protein band 4.1) genes, are components of the cortical cytoskeleton underlying the cell membrane. The family of 4.1 proteins consists of the eponymous 4.1R protein first identified in erythrocytes (gene: EPB41), 4.1N (EPB41L1), 4.1G (EPB41L2), 4.1B (EPB41L3) as well as the less closely related members NBL4 (EPB41L4A), EHM2 (EPB41L4B) and EPB41L5 (EPB41L5). EHM2 is conspicuous in tumor cells with high migratory potential, such as metastatic melanoma and fibrosarcoma cells. In prostate cancer, EHM2 has been reported to be overexpressed and to diminish adhesion of prostate cancer cells to collagen. (PMID: 20860828)