EPCAM/CD326 Monoklonaler Antikörper

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• KD/KO Validated

EPCAM/CD326 Monoklonal Antikörper für IF/ICC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IF/ICC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Publikationen(1)

CloneNo.

2A2D5

Kat-Nr. : CL488-66316

Synonyme

EPCAM, 2A2D5, Adenocarcinoma-associated antigen, CD326, Cell surface glycoprotein Trop-1

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  IF Staining of HT-29 using CL488-66316

  Immunofluorescent analysis of (-20°C Methanol) fixed HT-29 cells using CoraLite® Plus 488 EPCAM/CD326 antibody (CL488-66316, Clone: 2A2D5 ) at dilution of 1:200.

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  IF Staining of MCF-7 using CL488-66316

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 EPCAM/CD326 antibody (CL488-66316, Clone: 2A2D5 ) at dilution of 1:100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HT-29-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

CL488-66316 bindet in WB, IF/ICC EPCAM/CD326 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: EPCAM/CD326 fusion protein Ag15393
• Vollständiger Name: epithelial cell adhesion molecule
• Berechnetes Molekulargewicht: 314 aa, 35 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC014785
• Gene symbol: EPCAM
• Gene ID (NCBI): 4072
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. (PMID: 20837599; 19249674; 21576002; 22647938)

Publikationen

Species	Application	Title
human	WB	Cell Death Discov; MCM2 promotes the stemness and sorafenib resistance of hepatocellular carcinoma cells via hippo signaling; Authors - Xin Zhou