EPCAM/CD326 Monoklonaler Antikörper

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EPCAM/CD326 Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

2A2D5

Kat-Nr. : 66316-1-PBS

Synonyme

EPCAM, 2A2D5, Adenocarcinoma-associated antigen, CD326, Cell surface glycoprotein Trop-1

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  WB analysis of A431 using 66316-1-Ig (same clone as 66316-1-PBS)

  WB result of EPCAM antibody (66316-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPCAM transfected A431 cells. This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

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  WB analysis using 66316-1-Ig (same clone as 66316-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66316-1-Ig (EPCAM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

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  WB analysis of T-47D using 66316-1-Ig (same clone as 66316-1-PBS)

  T-47D, MCF-7 and A431 cells were subjected to SDS PAGE followed by western blot with 66316-1-Ig (EPCAM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

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  IHC staining of Human colon using 66316-1-Ig (same clone as 66316-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Human colon tissue slide using 66316-1-Ig (EPCAM/CD326 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66316-1-Ig (same clone as 66316-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66316-1-Ig (EPCAM Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

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  IF Staining of human stomach cancer using 66316-1-Ig (same clone as 66316-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human stomach cancer tissue using EPCAM/CD326 antibody (66316-1-Ig, Clone: 2A2D5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

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  IF Staining of human colon cancer using 66316-1-Ig (same clone as 66316-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using EPCAM/CD326 antibody (66316-1-Ig, Clone: 2A2D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

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  IF Staining of human colon cancer using 66316-1-Ig (same clone as 66316-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using EPCAM/CD326 antibody (66316-1-Ig, Clone: 2A2D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 66316-1-Ig (same clone as 66316-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using EPCAM antibody (66316-1-Ig, Clone: 2A2D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

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  IF Staining of HT-29 using 66316-1-Ig (same clone as 66316-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using EPCAM/CD326 antibody (66316-1-Ig, Clone: 2A2D5 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66316-1-PBS bindet in WB, IHC, IF/ICC, IF-P, ELISA EPCAM/CD326 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: EPCAM/CD326 fusion protein Ag15393
• Vollständiger Name: epithelial cell adhesion molecule
• Berechnetes Molekulargewicht: 314 aa, 35 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC014785
• Gene symbol: EPCAM
• Gene ID (NCBI): 4072
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)