EPCAM/CD326 Rekombinanter Antikörper

EPCAM/CD326 Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

241243B10

Kat-Nr. : 84073-6-RR

Synonyme

EPCAM, 241243B10, Adenocarcinoma-associated antigen, CD326, Cell surface glycoprotein Trop-1

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  WB analysis using 84073-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84073-6-RR (EPCAM/CD326 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 84073-6-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84073-6-RR (EPCAM/CD326 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 84073-6-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84073-6-RR (EPCAM/CD326 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 84073-6-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84073-6-RR (EPCAM/CD326 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 84073-6-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using EPCAM/CD326 antibody (84073-6-RR, Clone: 241243B10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84073-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84073-6-RR against Human EPCAM/CD326 were performed. The affinity constant is 2.56 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HaCaT-Zellen, HCT 116-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: human ovary cancer tissue, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

84073-6-RR bindet in WB, IHC, IF/ICC, ELISA EPCAM/CD326 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: EPCAM/CD326 fusion protein Eg1370
• Vollständiger Name: epithelial cell adhesion molecule
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: NM_002354.3
• Gene symbol: EPCAM
• Gene ID (NCBI): 4072
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)

Publikationen

Species	Application	Title
human	IF	J Transl Med; Tumor microenvironment remodeling after neoadjuvant chemoradiotherapy in local advanced rectal cancer revealed by single-cell RNA sequencing; Authors - Wenzhao Su