EPCAM/CD326 Rekombinanter Antikörper

EPCAM/CD326 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241851E11

Kat-Nr. : 84490-2-RR

Synonyme

Epithelial cell adhesion molecule, Ep-CAM, Epcam, EGP314, CD326

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  WB analysis using 84490-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84490-2-RR (EpCAM/TROP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 84490-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84490-2-RR (EPCAM/CD326 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 84490-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84490-2-RR (EPCAM/CD326 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 84490-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 84490-2-RR (EPCAM/CD326 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 84490-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 84490-2-RR (EPCAM/CD326 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84490-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84490-2-RR against Mouse EPCAM/CD326 were performed. The affinity constant is 0.779 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Kolongewebe, Maus-Dünndarmgewebe
• Erfolgreiche Detektion in IHC: Maus-Kolongewebe, Maus-Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84490-2-RR bindet in WB, IHC, ELISA EPCAM/CD326 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: epithelial cell adhesion molecule
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 35 kDa, 40 kDa
• GenBank-Zugangsnummer: NM_008532.2
• Gene symbol: Epcam
• Gene ID (NCBI): 17075
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)