EPCR/CD201 Rekombinanter Antikörper

EPCR/CD201 Rekombinant Antikörper für WB, IHC, IF/ICC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241853A9

Kat-Nr. : 84494-2-PBS

Synonyme

241853A9, Activated protein C receptor, APC receptor, CD201, Centrocyclin

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  WB analysis using 84494-2-RR (same clone as 84494-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84494-2-RR (EPCR/CD201 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84494-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP01323-3

  The mean PROCR concentration was determined to be 469,591.3 pg/mL in bEnd.3 cell extract based on a 1.8 mg/mL extract load and 30,645.7 pg/mL in mouse lung tissue extract based on a 1.6 mg/mL extract load.

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  Sandwich ELISA standard curve of MP01323-3

  Sandwich ELISA standard curve of MP01323-3, Mouse PROCR Recombinant Matched Antibody Pair - PBS only. 84494-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1517. 84494-3-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

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  IHC staining of mouse placenta using 84494-2-RR (same clone as 84494-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 84494-2-RR (EPCR/CD201 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84494-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse placenta using 84494-2-RR (same clone as 84494-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 84494-2-RR (EPCR/CD201 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84494-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse placenta using 84494-2-RR (same clone as 84494-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse placenta tissue using EPCR/CD201 antibody (84494-2-RR, Clone: 241853A9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84494-2-PBS in a different storage buffer formulation.

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  IF Staining of bEnd.3 using 84494-2-RR (same clone as 84494-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using EPCR/CD201 antibody (84494-2-RR, Clone: 241853A9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84494-2-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01323-3

  Cytometric bead array standard curve of MP01323-3, MOUSE EPCR Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84494-2-PBS. Detection antibody: 84494-3-PBS. Standard: Eg1517. Range: 0.469-60 ng/mL.

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Geprüfte Anwendungen

Produktinformation

84494-2-PBS bindet in WB, IHC, IF/ICC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA EPCR/CD201 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: EPCR/CD201 fusion protein Eg1517
• Vollständiger Name: protein C receptor, endothelial
• Berechnetes Molekulargewicht: 27kDa
• Beobachtetes Molekulargewicht: 40-50 kDa
• GenBank-Zugangsnummer: NM_011171.2
• Gene symbol: PROCR
• Gene ID (NCBI): 19124
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein C receptor (PROCR), also known as activated protein C receptor (APC receptor), EPCR, or CD201, is a transmembrane glycoprotein. EPCR promotes the activation of protein C, which has anticoagulant and cytoprotective effects. EPCR facilitates the activation of protein C by the thrombin-thrombomodulin complex but also facilitates APC-mediated cytoprotective impact on cells that involve the activation of protease-activated receptors (PAR) 1 and 3(PMID: 27207424).