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EPG5 Polyklonaler Antikörper

EPG5 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27666-1-PBS

Synonyme

HEEW1, KIAA1632, UPF0493 protein KIAA1632

Filter:
  • Western Blot (WB) analysis of various lysates using EPG5 Polyclonal antibody (27666-1-AP)

    WB analysis using 27666-1-AP (same clone as 27666-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27666-1-AP (EPG5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27666-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using EPG5 Polyclonal antibody (27666-1-AP)

    IHC staining of human kidney using 27666-1-AP (same clone as 27666-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27666-1-AP (KIAA1632 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 27666-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using EPG5 Polyclonal antibody (27666-1-AP)

    IHC staining of human kidney using 27666-1-AP (same clone as 27666-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27666-1-AP (KIAA1632 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 27666-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using EPG5 Polyclonal antibody (27666-1-AP)

    IHC staining of human lung cancer using 27666-1-AP (same clone as 27666-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27666-1-AP (EPG5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27666-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

27666-1-PBS bindet in WB, IHC, Indirect ELISA EPG5 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen EPG5 fusion protein Ag24801
Vollständiger Name KIAA1632
Berechnetes Molekulargewicht 2579 aa, 292 kDa
Beobachtetes Molekulargewicht270-290 kDa
GenBank-ZugangsnummerBC130614
Gene symbol EPG5
Gene ID (NCBI) 57724
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EPG5, a metazoan-specific autophagy protein, regulates autophagy by promoting fusion of autophagosomes with lysosomes and/or late endosomes. EPG5 regulates the stability and the assembly of STX17-SNAP29-VAMP8 trans-SNARE complexes. Loss of function of EPG5 causes non-specific fusion of autophagosomes with other endocytic vesicles, resulting in formation of non-degradative enlarged vesicles. EPG5 expression in human pluripotent stem cells like ESC and iPSC is higher than that of human somatic cells.(PMID: 30931944, PMID: 27588602)