EPHA1-specific Monoklonaler Antikörper

EPHA1-specific Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B7C3

Kat-Nr. : 60155-2-PBS

Synonyme

EPH, EPH receptor A1, EPHA1, EPHA1-specific, Ephrin type A receptor 1, EPHT, EPHT1

---

• 

  WB analysis of HeLa using 60155-2-Ig (same clone as 60155-2-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60155-2-Ig (EPHA1-specific antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60155-2-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 60155-2-Ig (same clone as 60155-2-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 60155-2-Ig (EPHA1-specific antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60155-2-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 60155-2-Ig (same clone as 60155-2-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60155-2-Ig (EPHA1-specific antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60155-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60155-2-Ig (same clone as 60155-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60155-2-Ig (EPHA1-specific antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60155-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60155-2-Ig (same clone as 60155-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60155-2-Ig (EPHA1-specific antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60155-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 60155-2-Ig (same clone as 60155-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60155-2-Ig (EPHA1-specific antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60155-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 60155-2-Ig (same clone as 60155-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60155-2-Ig (EPHA1-specific antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60155-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 60155-2-Ig (same clone as 60155-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60155-2-Ig (EPHA1-specific antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60155-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 60155-2-Ig (same clone as 60155-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60155-2-Ig (EPHA1-specific antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60155-2-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

60155-2-PBS bindet in WB, IHC, Indirect ELISA EPHA1-specific und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: EPH receptor A1
• Berechnetes Molekulargewicht: 108 kDa
• Beobachtetes Molekulargewicht: 108 kDa
• GenBank-Zugangsnummer: BC130291
• Gene symbol: EPHA1
• Gene ID (NCBI): 2041
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Caprylsäure/Ammoniumsulfat-Präzipitation
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EPHA1, also named as EPH, EPHT and EPHT1, belongs to ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. Ligands in the ephrin-A subclass, including the prototype family member ephrin-A1 (B61), are membrane associated through glycosylphosphatidyl-inositol linkages, whereas ephrin-B subclass consists of ligands with transmembrane domains. The general role of the Eph family is in mediating repulsive cell-cell interaction.