EPHA2 Monoklonaler Antikörper

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• KD/KO Validated

EPHA2 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

CloneNo.

1A9C3

Kat-Nr. : 66736-1-Ig

Synonyme

1A9C3, EC:2.7.10.1, ECK, EPH receptor A2, Ephrin type A receptor 2

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  WB analysis using 66736-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66736-1-Ig (EPHA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 66736-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EPHA2 antibody (66736-1-Ig, Clone: 1A9C3 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, BxPC-3-Zellen, HEK-293-Zellen, HepG2-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IHC: See 1 publications below

Produktinformation

66736-1-Ig bindet in WB, IHC, IF/ICC, ELISA EPHA2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: EPHA2 fusion protein Ag22566
• Vollständiger Name: EPH receptor A2
• Berechnetes Molekulargewicht: 976 aa, 108 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC037166
• Gene symbol: EPHA2
• Gene ID (NCBI): 1969
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EPHA2 (Ephrin type-A receptor 2) belongs to the receptor tyrosine kinase (RTK) family, with 16 known receptors and 9 known membrane-bound ligands in all species. Based on the extracellular domain sequence homology, structure, and binding affinity, Eph receptors and their ephrin ligands can be divided into A and B subtypes (PMID: 33962882). EPHA2 contains a conserved N-terminal ligand-bound extracellular domain, a transmembrane domain, and a conserved tyrosine kinase domain (PMID: 8070404).

Publikationen

Species	Application	Title
human	WB	Mol Ther Nucleic Acids; EFNA4 promotes cell proliferation and tumor metastasis in hepatocellular carcinoma through a PIK3R2/GSK3β/β-catenin positive feedback loop.; Authors - Junhao Lin
human	WB	Int J Mol Sci; Role of Nuclear Claudin-4 in Renal Cell Carcinoma.; Authors - Takuya Owari
mouse,human	WB	J Oncol; EphA2 Promotes the Development of Cervical Cancer through the CXCL11/PD-L1 Pathway; Authors - Xinyue Zhao ; KD Validated
mouse	WB	J Alzheimers Dis; Metabolic Reprogramming of Microglia Enhances Proinflammatory Cytokine Release through EphA2/p38 MAPK Pathway in Alzheimer's Disease; Authors - Xiaowei Ma ; KD Validated
human	WB	Chem Biol Interact; 2-Methoxy-5((3,4,5-trimethosyphenyl)seleninyl) phenol (SQ) inhibits cancer cell metastasis behavior of TNBC via suppressing EMT and VEGF.; Authors - Dayong Zheng
human	WB	Arch Biochem Biophys; EphA2 inhibits SRA01/04 cells apoptosis by suppressing autophagy via activating PI3K/Akt/mTOR pathway.; Authors - Jing Han ; KD Validated