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EPHA2 Monoklonaler Antikörper

EPHA2 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1A9C3

Kat-Nr. : 66736-1-PBS

Synonyme

1A9C3, EC:2.7.10.1, ECK, EPH receptor A2, Ephrin type A receptor 2

Filter:
  • Western Blot (WB) analysis of various lysates using EPHA2 Monoclonal antibody (66736-1-Ig)

    WB analysis using 66736-1-Ig (same clone as 66736-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66736-1-Ig (EPHA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66736-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EPHA2 Monoclonal antibody (66736-1-Ig)

    IF Staining of HepG2 using 66736-1-Ig (same clone as 66736-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EPHA2 antibody (66736-1-Ig, Clone: 1A9C3 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66736-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

66736-1-PBS bindet in WB, IF/ICC, ELISA EPHA2 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen EPHA2 fusion protein Ag22566
Vollständiger Name EPH receptor A2
Berechnetes Molekulargewicht 976 aa, 108 kDa
Beobachtetes Molekulargewicht 130 kDa
GenBank-ZugangsnummerBC037166
Gene symbol EPHA2
Gene ID (NCBI) 1969
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EPHA2 (Ephrin type-A receptor 2) belongs to the receptor tyrosine kinase (RTK) family, with 16 known receptors and 9 known membrane-bound ligands in all species. Based on the extracellular domain sequence homology, structure, and binding affinity, Eph receptors and their ephrin ligands can be divided into A and B subtypes (PMID: 33962882). EPHA2 contains a conserved N-terminal ligand-bound extracellular domain, a transmembrane domain, and a conserved tyrosine kinase domain (PMID: 8070404).