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EPHB3 Polyklonaler Antikörper

EPHB3 Polyklonal Antikörper für IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21382-1-PBS

Synonyme

EK2, EPH like kinase 2, EPH receptor B3, Ephrin type B receptor 3, ETK2

Filter:
  • Immunohistochemistry (IHC) staining of mouse brain tissue using EPHB3 Polyclonal antibody (21382-1-AP)

    IHC staining of mouse brain using 21382-1-AP (same clone as 21382-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21382-1-AP (EPHB3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21382-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using EPHB3 Polyclonal antibody (21382-1-AP)

    IHC staining of mouse brain using 21382-1-AP (same clone as 21382-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21382-1-AP (EPHB3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21382-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using EPHB3 Polyclonal antibody (21382-1-AP)

    IF Staining of Jurkat using 21382-1-AP (same clone as 21382-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using EPHB3 antibody (21382-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 21382-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

21382-1-PBS bindet in IHC, IF/ICC, Indirect ELISA EPHB3 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen EPHB3 fusion protein Ag16016
Vollständiger Name EPH receptor B3
Berechnetes Molekulargewicht 998 aa, 110 kDa
GenBank-ZugangsnummerBC052968
Gene symbol EPHB3
Gene ID (NCBI) 2049
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EPHB3 is a member of the Eph receptor tyrosine kinase family. The Eph receptors comprise a large family of closely related transmembrane tyrosine kinases that actively signal when bound to their ephrin ligands. The Eph receptors are characterized by an extracellular region with a unique cysteine-rich motif extending over its amino-terminal half, followed by two fibronectin type III motifs (PMID: 9530499). They are divided into two sub-groups (EphA and EphB) based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands (PMID: 11114742). The EPHB3 protein has multiple functions in the nervous system, including regulating neural networks, participating in epileptic activity, and playing a role in repair after spinal cord injury.