EPHB4 Polyklonaler Antikörper

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EPHB4 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20883-1-PBS

Synonyme

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• 

  WB analysis using 20883-1-AP (same clone as 20883-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 20883-1-AP (same clone as 20883-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 20883-1-AP (same clone as 20883-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HT-1080 using 20883-1-AP (same clone as 20883-1-PBS)

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.

• 

  WB analysis of DU 145 using 20883-1-AP (same clone as 20883-1-PBS)

  DU 145 cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.

• 

  WB analysis of DU 145 using 20883-1-AP (same clone as 20883-1-PBS)

  DU 145 cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 20883-1-AP (same clone as 20883-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 20883-1-AP (same clone as 20883-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat testis using 20883-1-AP (same clone as 20883-1-PBS)

  rat testis tissue were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat testis using 20883-1-AP (same clone as 20883-1-PBS)

  rat testis tissue were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 20883-1-AP (same clone as 20883-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20883-1-AP (EPHB4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 20883-1-AP (same clone as 20883-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20883-1-AP (EPHB4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 20883-1-AP (same clone as 20883-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas using 20883-1-AP (EPHB4 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 20883-1-AP (same clone as 20883-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas using 20883-1-AP (EPHB4 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 20883-1-AP (same clone as 20883-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 20883-1-AP (EPHB4 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 20883-1-AP (same clone as 20883-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 20883-1-AP (EPHB4 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 20883-1-AP (same clone as 20883-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 20883-1-AP (EPHB4 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 20883-1-AP (same clone as 20883-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 20883-1-AP (EPHB4 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HT-29 using 20883-1-AP (same clone as 20883-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using EPHB4 antibody (20883-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20883-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA EPHB4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EPHB4 fusion protein Ag10033
• Vollständiger Name: EPH receptor B4
• Berechnetes Molekulargewicht: 987 aa, 108 kDa
• Beobachtetes Molekulargewicht: 110-120 kDa
• GenBank-Zugangsnummer: BC052804
• Gene symbol: EPHB4
• Gene ID (NCBI): 2050
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.