EPHX1 Monoklonaler Antikörper

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EPHX1 Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B6A2

Kat-Nr. : 66982-1-PBS

Synonyme

EPHX, EPHX1, EPOX, Epoxide hydratase, Epoxide hydrolase 1, MEH, Microsomal epoxide hydrolase

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  WB analysis using 66982-1-Ig (same clone as 66982-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66982-1-Ig (EPHX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66982-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66982-1-Ig (same clone as 66982-1-PBS)

  WB result of EPHX1 antibody (66982-1-Ig; 1:120000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPHX1 transfected HeLa cells. This data was developed using the same antibody clone with 66982-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 66982-1-Ig (same clone as 66982-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 66982-1-Ig (EPHX1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66982-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 66982-1-Ig (same clone as 66982-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 66982-1-Ig (EPHX1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66982-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66982-1-PBS bindet in WB, Indirect ELISA EPHX1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: EPHX1 fusion protein Ag25820
• Vollständiger Name: epoxide hydrolase 1, microsomal (xenobiotic)
• Berechnetes Molekulargewicht: 53 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC003567
• Gene symbol: EPHX1
• Gene ID (NCBI): 2052
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EPHX1, also known as EPHX and EPOX, belongs to the peptidase S33 family. EPHX1 is an evolutionarily highly conserved biotransformation enzyme expressed in nearly all tissues and localized mainly in the microsomal fraction of the endoplasmic reticulum of eukaryotic cells. EPHX1 plays a role in the metabolism of endogenous lipids such as epoxide-containing fatty acids. The calculated molecular weight of EPHX1 is 53 kDa (PMID: 26216302).