EPHX2 Rekombinanter Antikörper

EPHX2 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

251753F4

Kat-Nr. : 86790-1-RR

Synonyme

Bifunctional epoxide hydrolase 2, CEH, Cytosolic epoxide hydrolase 2, Lipid-phosphate phosphatase, SEH

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  WB analysis using 86790-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86790-1-RR (EPHX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U-937-Zellen, A549-Zellen, HEK-293-Zellen, Jurkat-Zellen, LNCaP-Zellen, Maus-Kolongewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86790-1-RR bindet in WB, ELISA EPHX2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: EPHX2 fusion protein Ag1283
• Vollständiger Name: epoxide hydrolase 2, cytoplasmic
• Berechnetes Molekulargewicht: 63 kDa
• Beobachtetes Molekulargewicht: 57 kDa
• GenBank-Zugangsnummer: BC013874
• Gene symbol: EPHX2
• Gene ID (NCBI): 2053
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EPHX2(Epoxide hydrolase 2) acts on epoxides (alkene oxides, oxiranes) and arene oxides and plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. A number of single nucleotide polymorphisms (SNPs) in human EPHX2 have been linked to cardiovascular disease risk, including increased risk of coronary heart disease, hyperlipoproteinemia, and type-2 diabetes (PMID: 14732757, 16595607, 14673705, 15845398, 17460077). It was observed in many tissues with the band of 63 kDa in the western blot. It has also been reported that the N-terminal domain might promote dimerization of EPHX2 (PMID:21553642). Negative expression of EPHX2 protein in U-937 cells is consistent with the predicted expression pattern.