EPLIN Monoklonaler Antikörper

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• KD/KO Validated

EPLIN Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

6E6E6

Kat-Nr. : 66071-1-PBS

Synonyme

LIMA1, 6E6E6, LIM domain and actin binding 1, LIM domain and actin-binding protein 1, PP624

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  WB analysis of HEK-293 using 66071-1-Ig (same clone as 66071-1-PBS)

  WB result of EPLIN antibody (66071-1-Ig, 1:2000) with si-Control and si-EPLIN transfected HEK293 cells. This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

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  WB analysis using 66071-1-Ig (same clone as 66071-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

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  WB analysis using 66071-1-Ig (same clone as 66071-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66071-1-Ig (same clone as 66071-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 66071-1-Ig (same clone as 66071-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66071-1-Ig (same clone as 66071-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 66071-1-Ig (same clone as 66071-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66071-1-Ig (EPLIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 66071-1-Ig (same clone as 66071-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 66071-1-Ig(EPLIN antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 66071-1-Ig (same clone as 66071-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 66071-1-Ig(EPLIN antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66071-1-Ig (same clone as 66071-1-PBS)

  Immunofluorescent analysis of Hela cells using 66071-1-Ig (EPLIN antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66071-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66071-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA EPLIN und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: EPLIN fusion protein Ag13288
• Vollständiger Name: LIM domain and actin binding 1
• Berechnetes Molekulargewicht: 85 kDa
• Beobachtetes Molekulargewicht: 90 kDa, 110 kDa
• GenBank-Zugangsnummer: BC001247
• Gene symbol: EPLIN
• Gene ID (NCBI): 51474
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EPLIN is a cytoskeletal protein that is preferentially expressed in epithelial cells and is implicated in regulation of cytoskeletal dynamics and cytokinesis. In human, there are two known isoforms, EPLIN-a and -b, generated by alternative promoter usage from a single gene. EPLIN-a expression is often down-regulated in cancerous cells and tissues. This antibody recognizes both of isoforms of EPLN.