EPO/Erythropoietin Polyklonaler Antikörper

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• KD/KO Validated

EPO/Erythropoietin Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 17908-1-AP

Synonyme

EPO, MVCD2, erythropoietin, Epoetin, EP

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  WB analysis of HEK-293 using 17908-1-AP

  WB result of EPO/Erythropoietin antibody (17908-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPO transfected HEK-293 cells.

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  IHC staining of rat kidney using 17908-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 17908-1-AP (EPO/Erythropoietin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 17908-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 17908-1-AP (EPO/Erythropoietin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: Rattennierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 2 publications below

Produktinformation

17908-1-AP bindet in WB, IHC, ELISA EPO/Erythropoietin und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Hund, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EPO/Erythropoietin fusion protein Ag12302
• Vollständiger Name: erythropoietin
• Berechnetes Molekulargewicht: 193 aa, 21 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC093628
• Gene symbol: EPO/Erythropoietin
• Gene ID (NCBI): 2056
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Erythropoietin (Epo) is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. Erythropoietin (Epo) is a 166 amino acids protein containing three N-glycosylation sites (Asn-24, Asn-38, and Asn-83) and 1 O- glycosylation site (Ser-126) and involved in the regulation of the level of red blood cells. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. Its effect is realized by binding erythropoietin receptor (EpoR) expressed on erythroid progenitor cells. EpoR, is a glycoprotein expressed on megakaryocytes, erythroid progenitors and endothelial cells. Epo also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.

Publikationen

Species	Application	Title
human	IHC	Front Genet; Identification and Validation of a Tumor Microenvironment-Related Gene Signature in Hepatocellular Carcinoma Prognosis.; Authors - Changjing Huang
canine	IHC	J Vet Med Sci; Suspected renal interstitial cell tumor causing polycythemia in two dogs; Authors - Kaori Ide
rat	WB	Front Pharmacol; Dimethyloxalylglycine pretreatment of living donor alleviates both donor and graft liver ischemia-reperfusion injury in rats; Authors - Degong Jia
human	WB	Int J Clin Oncol; The miR-199a-5p/HIF1α dual-regulatory axis participates in hypoxia-induced aggressive phenotypes of oral squamous cell carcinoma (OSCC) cells; Authors - Xing Chen
human	WB	Front Mol Neurosci; The cytokine receptor CRLF3 is a human neuroprotective EV-3 (Epo) receptor; Authors - Debbra Y Knorr