EPO/Erythropoietin Monoklonaler Antikörper

EPO/Erythropoietin Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1A11F3

Kat-Nr. : 66975-1-PBS

Synonyme

EPO, MVCD2, erythropoietin, Epoetin, EP

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  WB analysis of Recombinant protein using 66975-1-Ig (same clone as 66975-1-PBS)

  50 ng recombinant human EPO (cat.NO. HZ-1168) was subjected to SDS-PAGE under reducing condition followed by western blot with 66975-1-Ig (EPO antibody) at dilution of 1:10000. This data was developed using the same antibody clone with 66975-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66975-1-Ig (same clone as 66975-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66975-1-Ig (EPO/Erythropoietin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66975-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66975-1-Ig (same clone as 66975-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66975-1-Ig (EPO/Erythropoietin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66975-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66975-1-Ig (same clone as 66975-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EPO antibody (66975-1-Ig, Clone: 1A11F3 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66975-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66975-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA EPO/Erythropoietin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: EPO/Erythropoietin fusion protein Ag23054
• Vollständiger Name: erythropoietin
• Berechnetes Molekulargewicht: 193 aa, 21 kDa
• GenBank-Zugangsnummer: BC093628
• Gene symbol: EPO/Erythropoietin
• Gene ID (NCBI): 2056
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Erythropoietin (Epo) is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. Its effect is realized by binding erythropoietin receptor (EpoR) expressed on erythroid progenitor cells. EpoR, is a glycoprotein expressed on megakaryocytes, erythroid progenitors and endothelial cells. Epo also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.