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EPO/Erythropoietin Polyklonaler Antikörper

EPO/Erythropoietin Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17908-1-PBS

Synonyme

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  • Western Blot (WB) analysis of HEK-293 cells using EPO/Erythropoietin Polyclonal antibody (17908-1-AP)

    WB analysis of HEK-293 using 17908-1-AP (same clone as 17908-1-PBS)

    WB result of EPO/Erythropoietin antibody (17908-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPO transfected HEK-293 cells. This data was developed using the same antibody clone with 17908-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using EPO/Erythropoietin Polyclonal antibody (17908-1-AP)

    IHC staining of rat kidney using 17908-1-AP (same clone as 17908-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 17908-1-AP (EPO/Erythropoietin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17908-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using EPO/Erythropoietin Polyclonal antibody (17908-1-AP)

    IHC staining of rat kidney using 17908-1-AP (same clone as 17908-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 17908-1-AP (EPO/Erythropoietin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17908-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

17908-1-PBS bindet in WB, IHC, Indirect ELISA EPO/Erythropoietin und zeigt Reaktivität mit human, Ratten

Getestete Reaktivität human, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen EPO/Erythropoietin fusion protein Ag12302
Vollständiger Name erythropoietin
Berechnetes Molekulargewicht 193 aa, 21 kDa
Beobachtetes Molekulargewicht 37 kDa
GenBank-ZugangsnummerBC093628
Gene symbol EPO/Erythropoietin
Gene ID (NCBI) 2056
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Erythropoietin (Epo) is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. Erythropoietin (Epo) is a 166 amino acids protein containing three N-glycosylation sites (Asn-24, Asn-38, and Asn-83) and 1 O- glycosylation site (Ser-126) and involved in the regulation of the level of red blood cells. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. Its effect is realized by binding erythropoietin receptor (EpoR) expressed on erythroid progenitor cells. EpoR, is a glycoprotein expressed on megakaryocytes, erythroid progenitors and endothelial cells. Epo also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.