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EPS15 Polyklonaler Antikörper

EPS15 Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31301-1-PBS

Synonyme

AF 1P, AF1P, MLLT5, Protein AF 1p, Protein Eps15

Filter:
  • Western Blot (WB) analysis of various lysates using EPS15 Polyclonal antibody (31301-1-AP)

    WB analysis using 31301-1-AP (same clone as 31301-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31301-1-AP (EPS15 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31301-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using EPS15 Polyclonal antibody (31301-1-AP)

    WB analysis of MCF-7 using 31301-1-AP (same clone as 31301-1-PBS)

    WB result of EPS15 antibody (31301-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPS15 transfected MCF-7 cells. This data was developed using the same antibody clone with 31301-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

31301-1-PBS bindet in WB, Indirect ELISA EPS15 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen EPS15 fusion protein Ag35282
Vollständiger Name epidermal growth factor receptor pathway substrate 15
Berechnetes Molekulargewicht99 kDa
Beobachtetes Molekulargewicht140 kDa
GenBank-ZugangsnummerNM_001981.3
Gene symbol EPS15
Gene ID (NCBI) 2060
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EPS15 was originally identified as a substrate for the kinase activity of the epidermal growth factor receptor (EGFR). EPS15 has a tripartite structure comprising an NH2-terminal portion, which contains three EH domains, a central putative coiled-coil region, and a COOH-terminal domain containing multiple copies of the amino acid triplet Aspartate-Proline-Phenylalanine (PMID: 10481267). EPS15 is phosphorylated on tyrosine residues following EGFR activation (PMID: 7689153).