EPS15R Polyklonaler Antikörper

EPS15R Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21243-1-PBS

Synonyme

EPS15L1, Eps15-related protein, Eps15 related protein, Epidermal growth factor receptor substrate 15-like 1

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  WB analysis using 21243-1-AP (same clone as 21243-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21243-1-AP (EPS15R antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21243-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 21243-1-AP (same clone as 21243-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21243-1-AP (EPS15R antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21243-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 21243-1-AP (same clone as 21243-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 21243-1-AP (EPS15R antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21243-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 21243-1-AP (same clone as 21243-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 21243-1-AP (EPS15R antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21243-1-PBS in a different storage buffer formulation.

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  IF Staining of BxPC-3 using 21243-1-AP (same clone as 21243-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed BxPC-3 cells using 21243-1-AP (EPS15R antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21243-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

21243-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA EPS15R und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EPS15R fusion protein Ag15735
• Vollständiger Name: epidermal growth factor receptor pathway substrate 15-like 1
• Berechnetes Molekulargewicht: 864 aa, 94 kDa
• Beobachtetes Molekulargewicht: 94 kDa
• GenBank-Zugangsnummer: BC142716
• Gene symbol: EPS15R
• Gene ID (NCBI): 58513
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EPS15R is a protein that belongs to the Eps15 family of proteins, which are known for their roles in intracellular trafficking, particularly in the regulation of endocytosis and vesicle formation. EPS15R, like other members of the Eps15 family, functions as a scaffolding adaptor protein and is involved in both secretion and endocytosis. It has been shown to bind to AP-1 and AP-2 complexes, inositol lipids, and several other proteins that are crucial for the regulation of intracellular trafficking. EPS15R is also implicated in the regulation of membrane morphology, which is essential for intracellular vesicle formation and trafficking . It has been detected in the nucleus of mammalian cells, and its activation through growth factor receptors induces tyrosine phosphorylation and mono-ubiquitination, although the specific roles of these post-translational modifications are not yet fully understood