EPS8 Rekombinanter Antikörper

EPS8 Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242835G12

Kat-Nr. : 85184-5-PBS

Synonyme

Epidermal growth factor receptor kinase substrate 8

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  WB analysis using 85184-5-RR (same clone as 85184-5-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 85184-5-RR (EPS8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85184-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 85184-5-RR (same clone as 85184-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 85184-5-RR (EPS8 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85184-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 85184-5-RR (same clone as 85184-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 85184-5-RR (EPS8 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85184-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 85184-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85184-5-RR against Human EPS8 were performed. The affinity constant is 27.9 nM.

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Geprüfte Anwendungen

Produktinformation

85184-5-PBS bindet in WB, IHC, Indirect ELISA EPS8 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: EPS8 fusion protein Ag3121
• Vollständiger Name: epidermal growth factor receptor pathway substrate 8
• Berechnetes Molekulargewicht: 822 aa, 92 kDa
• Beobachtetes Molekulargewicht: 97 kDa
• GenBank-Zugangsnummer: BC030010
• Gene symbol: EPS8
• Gene ID (NCBI): 2059
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Epidermal growth factor receptor Pathway Substrate 8 (EPS8) is a crucial regulator of the actin cytoskeleton dynamics accompanying cell motility and invasion. This protein contains one PH domain and one SH3 domain leading to its binding activity with multiple cellular targets. EPS8 can function as a unique actin capping protein specifically required for dendritic cell migration and plays roles in the regulation of axonal filopodia in neuronal development and synapse formation. The EPS8 gene may contribute to the development of a subset of colorectal cancers and could have applications in diagnosis and treatment.