EPS8 Rekombinanter Antikörper

EPS8 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242835G12

Kat-Nr. : 85184-5-RR

Synonyme

Epidermal growth factor receptor kinase substrate 8

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  WB analysis using 85184-5-RR

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 85184-5-RR (EPS8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 85184-5-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 85184-5-RR (EPS8 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 85184-5-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 85184-5-RR (EPS8 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 85184-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85184-5-RR against Human EPS8 were performed. The affinity constant is 27.9 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, C2C12-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85184-5-RR bindet in WB, IHC, ELISA EPS8 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: EPS8 fusion protein Ag3121
• Vollständiger Name: epidermal growth factor receptor pathway substrate 8
• Berechnetes Molekulargewicht: 822 aa, 92 kDa
• Beobachtetes Molekulargewicht: 97 kDa
• GenBank-Zugangsnummer: BC030010
• Gene symbol: EPS8
• Gene ID (NCBI): 2059
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Epidermal growth factor receptor Pathway Substrate 8 (EPS8) is a crucial regulator of the actin cytoskeleton dynamics accompanying cell motility and invasion. This protein contains one PH domain and one SH3 domain leading to its binding activity with multiple cellular targets. EPS8 can function as a unique actin capping protein specifically required for dendritic cell migration and plays roles in the regulation of axonal filopodia in neuronal development and synapse formation. The EPS8 gene may contribute to the development of a subset of colorectal cancers and could have applications in diagnosis and treatment.