ER Rekombinanter Antikörper

ER Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241825D5

Kat-Nr. : 84564-4-PBS

Synonyme

ESR1, 241825D5, ER alpha, Era, ER-alpha

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  WB analysis using 84564-4-RR (same clone as 84564-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84564-4-RR (ER antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84564-4-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 84564-4-RR (same clone as 84564-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84564-4-RR (ER antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84564-4-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 84564-4-RR (same clone as 84564-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 84564-4-RR (ER antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84564-4-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 84564-4-RR (same clone as 84564-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 84564-4-RR (ER antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84564-4-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 84564-4-RR (same clone as 84564-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ER antibody (84564-4-RR, Clone: 241825D5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84564-4-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

84564-4-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA ER und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ER fusion protein Eg2234
• Vollständiger Name: estrogen receptor 1
• Berechnetes Molekulargewicht: 595 aa, 66 kDa
• Beobachtetes Molekulargewicht: 66 kDa
• GenBank-Zugangsnummer: BC128573
• Gene symbol: ER
• Gene ID (NCBI): 2099
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The estrogen receptor (ESR, ER) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. ESR1, also known as ESR or NR3A1, belongs to the nuclear hormone receptor family and NR3 subfamily. It is a nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. ESR1 can activate the transcriptional activity of TFF1.[PMID: 11731608,10970861]