ER Rekombinanter Antikörper

ER Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241825D5

Kat-Nr. : 84564-4-RR

Synonyme

ESR1, 241825D5, ER alpha, Era, ER-alpha

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  WB analysis using 84564-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84564-4-RR (ER antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 84564-4-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84564-4-RR (ER antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 84564-4-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 84564-4-RR (ER antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 84564-4-RR

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 84564-4-RR (ER antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 84564-4-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ER antibody (84564-4-RR, Clone: 241825D5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, T-47D-Zellen
• Erfolgreiche Detektion in IHC: human ovary cancer tissue, humanes Mammakarzinomgewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84564-4-RR bindet in WB, IHC, IF/ICC, ELISA ER und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ER fusion protein Eg2234
• Vollständiger Name: estrogen receptor 1
• Berechnetes Molekulargewicht: 595 aa, 66 kDa
• Beobachtetes Molekulargewicht: 66 kDa
• GenBank-Zugangsnummer: BC128573
• Gene symbol: ER
• Gene ID (NCBI): 2099
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The estrogen receptor (ESR, ER) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. ESR1, also known as ESR or NR3A1, belongs to the nuclear hormone receptor family and NR3 subfamily. It is a nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. ESR1 can activate the transcriptional activity of TFF1.[PMID: 11731608,10970861]