ERCC1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ERCC1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Kat-Nr. : 14586-1-AP

Synonyme

COFS4, ERCC1, UV20

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Galerie der Validierungsdaten

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  WB analysis using 14586-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14586-1-AP (ERCC1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 14586-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 14586-1-AP (ERCC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 14586-1-AP

  IP Result of anti-ERCC1 (IP:14586-1-AP, 3ug; Detection:14586-1-AP 1:300) with MCF-7 cells lysate 1600ug.

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  IHC staining of human testis using 14586-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14586-1-AP (ERCC1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 14586-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14586-1-AP (ERCC1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 14586-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14586-1-AP (ERCC1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 14586-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14586-1-AP (ERCC1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 14586-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14586-1-AP (ERCC1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 14586-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14586-1-AP (ERCC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 14586-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14586-1-AP (ERCC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 14586-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14586-1-AP (ERCC1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 14586-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14586-1-AP (ERCC1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 14586-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14586-1-AP (ERCC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 14586-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using 14586-1-AP (ERCC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Nierengewebe, MCF-7-Zellen, SKOV-3-Zellen, T-47D-Zellen
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Eierstockgewebe, humanes Hautgewebe, humanes Hirngewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 13 publications below
• IHC: See 3 publications below

Produktinformation

14586-1-AP bindet in WB, IP, IHC, IF, ELISA ERCC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ERCC1 fusion protein Ag6112
• Vollständiger Name: excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence)
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC052813
• Gene symbol: ERCC1
• Gene ID (NCBI): 2067
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Excision Repair Cross Complementing 1 (ERCC1) is a structure-specific endonuclease that is responsible for the 5'-incision during DNA repair. It forms a complex with ERCC11, XPF and ERCC4, which are required in both recombinatorial repair and nucleotide excision repair. It has been found that ERCC1, together with RRM1, are determinants of survival after surgical treatment of early-stage, non-small-cell lung cancer. (Ref: Simon, ER. 2005). The calculated molecular weight of ERCC1 is 33 kDa, but the modified ERCC1 is about38 kDa.

Publikationen

Species	Application	Title
human	WB	Blood Cancer J; Severe ineffective erythropoiesis discriminates prognosis in myelodysplastic syndromes: analysis based on 776 patients from a single centre.; Authors - Huijun Huang ; KD Validated
human	WB	Cell Death Dis; PFKFB3 blockade inhibits hepatocellular carcinoma growth by impairing DNA repair through AKT.; Authors - Wen-Kai Shi
human	WB	J Cell Mol Med; Berberine attenuates XRCC1-mediated base excision repair and sensitizes breast cancer cells to the chemotherapeutic drugs.; Authors - Xingjie Gao
human	WB	Oncotarget; Functional characterization of a novel transcript of ERCC1 in chemotherapy resistance of ovarian cancer.; Authors - Jia Liu
human	WB, IHC	Oncol Res; The AKT/GSK3-Mediated Slug Expression Contributes to Oxaliplatin Resistance in Colorectal Cancer via Upregulation of ERCC1.; Authors - Wei Wei ; KD Validated
human	WB	Cancer Cell Int; Mechanistic study of lncRNA UCA1 promoting growth and cisplatin resistance in lung adenocarcinoma.; Authors - Jiali Fu