• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ERCC1 Monoklonaler Antikörper

ERCC1 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1H4D5

Kat-Nr. : 66275-1-PBS

Synonyme

COFS4, ERCC1, UV20

Filter:
  • Western Blot (WB) analysis of A431 cells using ERCC1 Monoclonal antibody (66275-1-Ig)

    WB analysis of A431 using 66275-1-Ig (same clone as 66275-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 66275-1-Ig (ERCC1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66275-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ERCC1 Monoclonal antibody (66275-1-Ig)

    IF Staining of A431 using 66275-1-Ig (same clone as 66275-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed A431 cells using 66275-1-Ig(ERCC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66275-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

66275-1-PBS bindet in WB, IF/ICC, Indirect ELISA ERCC1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen ERCC1 fusion protein Ag6417
Vollständiger Name excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence)
Berechnetes Molekulargewicht 33 kDa
Beobachtetes Molekulargewicht 38 kDa
GenBank-ZugangsnummerBC052813
Gene symbol ERCC1
Gene ID (NCBI) 2067
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Excision Repair Cross Complementing 1 (ERCC1) is a structure-specific endonuclease that is responsible for the 5'-incision during DNA repair. It forms a complex with ERCC11, XPF and ERCC4, which are required in both recombinatorial repair and nucleotide excision repair. It has been found that ERCC1, together with RRM1, are determinants of survival after surgical treatment of early-stage, non-small-cell lung cancer. (Ref: Simon, ER. 2005)