ERCC2 Polyklonaler Antikörper

ERCC2 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10818-1-PBS

Synonyme

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  WB analysis of HeLa using 10818-1-AP (same clone as 10818-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10818-1-AP (ERCC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10818-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 10818-1-AP (same clone as 10818-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 10818-1-AP (ERCC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10818-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 10818-1-AP (same clone as 10818-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10818-1-AP (ERCC2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10818-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 10818-1-AP (same clone as 10818-1-PBS)

  IP result of anti-ERCC2 (IP:10818-1-AP, 4ug; Detection:10818-1-AP 1:1000) with HEK-293 cells lysate 1575 ug. This data was developed using the same antibody clone with 10818-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 10818-1-AP (same clone as 10818-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10818-1-AP (ERCC2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10818-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 10818-1-AP (same clone as 10818-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10818-1-AP (ERCC2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10818-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 10818-1-AP (same clone as 10818-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10818-1-AP (ERCC2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10818-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 10818-1-AP (same clone as 10818-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10818-1-AP (ERCC2 antibody) at dilution of 1:25 (under 10x lens). This data was developed using the same antibody clone with 10818-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 10818-1-AP (same clone as 10818-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10818-1-AP (ERCC2 antibody) at dilution of 1:25 (under 40x lens). This data was developed using the same antibody clone with 10818-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10818-1-PBS bindet in WB, IHC, IP, Indirect ELISA ERCC2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ERCC2 fusion protein Ag1038
• Vollständiger Name: excision repair cross-complementing rodent repair deficiency, complementation group 2
• Berechnetes Molekulargewicht: 87 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: BC008346
• Gene symbol: ERCC2
• Gene ID (NCBI): 2068
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Excision-repair complementing defective in Chinese hamster 2(ERCC2), belongs to the nucleotide excision repair pathway, is a component of the TFIIH transcription complex and required for the association of the CDK-activating kinase(CAK) subcomplex with TFIIH. Since TFIIH is involved in both DNA repair and cell cycle progression, ERCC2 is implicated to play an integral role in the nucleotide excision repair pathway. Also it involves in the regulation of vitamin-D receptor activity. As a part of the mitotic spindle-associated MMXD complex, ERCC2 has a role in chromosome segregation.