ERCC3 Polyklonaler Antikörper

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• KD/KO Validated

ERCC3 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10580-1-PBS

Synonyme

EC:5.6.2.4, DNA repair protein complementing XP-B cells, DNA excision repair protein ERCC-3, DNA 3'-5' helicase/translocase XPB, BTF2 p89

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  WB analysis of COLO 320 using 10580-1-AP (same clone as 10580-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10580-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 10580-1-AP (same clone as 10580-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10580-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 10580-1-AP (same clone as 10580-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10580-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 10580-1-AP (same clone as 10580-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10580-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 10580-1-AP (same clone as 10580-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10580-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse colon using 10580-1-AP (same clone as 10580-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10580-1-PBS in a different storage buffer formulation.

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  WB analysis of HT-1080 using 10580-1-AP (same clone as 10580-1-PBS)

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10580-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 10580-1-AP (same clone as 10580-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10580-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 10580-1-AP (same clone as 10580-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10580-1-AP (ERCC3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10580-1-PBS in a different storage buffer formulation.

• 

  IP experiment of A431 using 10580-1-AP (same clone as 10580-1-PBS)

  IP result of anti-ERCC3 (IP:10580-1-AP, 4ug; Detection:10580-1-AP 1:500) with A431 cells lysate 1200ug. This data was developed using the same antibody clone with 10580-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 10580-1-AP (same clone as 10580-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10580-1-AP (ERCC3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10580-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10580-1-PBS bindet in WB, IHC, IP, Indirect ELISA ERCC3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ERCC3 fusion protein Ag0876
• Vollständiger Name: excision repair cross-complementing rodent repair deficiency, complementation group 3 (xeroderma pigmentosum group B complementing)
• Berechnetes Molekulargewicht: 89 kDa
• Beobachtetes Molekulargewicht: 89 kDa
• GenBank-Zugangsnummer: BC008820
• Gene symbol: ERCC3
• Gene ID (NCBI): 2071
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Excision repair cross-complementing 3/xeroderma pigmentosum B (ERCC3/XPB) DNA helicase, is an essential ATP-dependent DNA helicase that functions as an integral component of the transcription factor IIH complex. ERCC3 is involved in initiation of basal transcription and global genome repair as well as in transcription-coupled repair (TCR) [PMID:19942596]. At the cellular level, a defect in ERCC3 results in high UV sensitivity, reduced post-UV RNA synthesis recovery and decreased DNA repair due to defective NER [PMID:16947863].